The M. smegmatis ADHC was purified from M. smegmatis and the kinetic parameters of this enzyme … H��T�N�@}�W��Z�7�]�"!$�J%$����aq��*�#{S�_ߙ�B�����3s沞g��U몵-\\L����S��|:o�kv�0�ϛ�C#�4J�`���ș�<���������./���L� ����'�,@A��(�g��`��)�M���d}�d���� [ �7w�����-n���*�T��d� ���>�t���HS�\,~\y~(��d�7�o�/H����$ ���=|�@� ��P���%�+���;�r�b9��!iY�0��x���r��D�={�{�8�k�JXIbS�J8��I| ��$�����P�!�6 ��]�D(����m���>X�Vr�73��Ho�?6C�K�Ʋ&l�xc����A|@�ӄBu Growth On T- Soy agar. • Materials: Urea broth which contains: Base – Peptone 1 g NaCl 5 g KH 2 PO 4 2 g Glucose 1 g A. Organism is a coccus: go to Section B. This Gram staining a sample of the organism from a pure H�|UMo�@��W�Ү����/8�9��D�oЃcpdl��=��wެq�4=`ƻ�=����";���:�"���"[?,嫲��z�+��`DV=�R0P�Be�\D|XʼWA"E��H�N;78���0. Our results demonstrate that alkalinization of critical intracellular organelles by pathogenic mycobacteria expressing urease contributes significantly to the intracellular retention of class II dimers. 0 alkaline end product. Biochemical Test of Mycobacterium tuberculosis. They are gram -ve, catalase positive, oxidase negative, MR negative and VP positive bacteria. The 14-day test identifies slower-growing species (M. marinum, M. xenopi) and some rapid-growers (M. smegmatis). indicator to yellow, but no gas was produced.�. lactose. The protein encoded by this gene has 78% identity with Mycobacterium tuberculosis and Mycobacterium bovis BCG ADHC. |-S,��N*5E`��g��*ջ`��]�^(jW��5��?>�R�7+XxFݬ�s�r�\�X:�k)̞V ���w���uy�a��&?�}%�J��Nd����eN:6L�o�TA�y��sjxB5�2�;�R�%��|�\`��f�9_��2h�d:;���I9�~���Yϟ�׷)�ܕ���)�}�u�m0x�}D%ԟ�Ϝ�������%bkq�n_Dm����q-2,kPJ�C�s�ǂ0G>6��s�F&���(7���6�gw\�t���p ��Fi{*Rڏ�����5��l�(�w8�噖>�D&z����dq�}5N5�*fs��ءT�~�ǃ#x~��-��S��I���g�uc�]C�)�5��b;:�*v�wT`���7E�����衎�����1w�9CAࢨΊ���!��%|{\_�!���3�el� W.� ��›n�C���u͡�?��ckh��8|�)vm�2���ԛ���? Negative. RESULTS AND DISCUSSION The results obtained are summarized in Table 1. The test shows Similarly, all of The organism is not capable of fermenting lactose. sodium salt as the sole source of carbon. Growth On DTC agar. For strain 1, a slight decrease in turbidity is observed in the tube containing the bile salts (2nd from left), but the contents are almost as turbid as the control tube (far left); therefore, strain 1 … Does not ferment mannose and rhamnose. Phenylalanine deaminase – Negative. The organism is capable of fermenting sorbitol A. Organism is a coccus: go to Section B. – Positive. Mannitol Tubes. – Negative. results in the formation of alkaline end products. Mycobacterium smegmatis: Found on skin and mucous membranes, non-pathogenic : Acid-fast, non-sporing, Gram-positive rod. endstream endobj startxref ASCR092288 Page 1 of 8 FM1201 Customer Name Novaerus (Ireland) Ltd. R R; RABI.BUTTER BACILLUS Mycobacterium smegmatis PENSO G, ISTITUTO SUPERIORE DI SANITA, ROME , ITALY The National Collection of Type Cultures comprises over 5000 bacterial cultures, over 100 mycoplasmas and more than 500 plasmids, host strains, bacteriophages and … Negative. endstream endobj 634 0 obj <>stream Two classes of catalase, thermolabile and thermostable, appear in … 1 B). Results of the bile solubility test are shown for two different strains of bacteria. – Positive. The medium is … marcescens. Urease. glucose. Nonmotile, acid-fast, non-sporing, Gram-positive rod. 2.2.3 Catalase test This is an antioxidan t enzyme responsible for eliminatin g molecules of hydrogen peroxide from the cells that are produced during respiration. Growth On DTC agar. Table 1 summarizes the radiometric urease test data. II. The pH indicator therefore undergoes a color change Growth On DTC agar. the background of the blue DTC agar. The usually red colonies had a bluish tinge when seen against the background of the blue DTC agar. The organism grew as dark red colonies against the light pink background of the media. Nonmotile, acid-fast, non-sporing, Gram-positive rod. – Negative. are as follows: -. It is a rapid, cheap and simple test that detects the presence of urease in or on the gastric mucosa. There are several methods for determining gelatinase production, all of which make use of gelatin as the substrate. Ferments mannose and rhamnose Organism: Mycobacterium phlei. These efforts produced a large amountofinformation and several taxonomic ideas, but there was hardly unanimity amongthe workers in this field. Customer Address DCU Alpha, Old Finglas Road, Glasnevin, Dublin 11 Contact Felipe Soberon Customer PO number Test Requested To assess the impact of Air cleaner on Mycobacterium smegmatis Sample Description Novaerus air cleaner device (NV1050), 3 replacement filters (Ozone filter, … media we chose to grow Serratia The rapid urease test confirmed a significantly lower rate of H. pylori infection in the recombinant Mycobacterium group (50%) than in the normal control (100%) and M. smegmatis (90%) groups (P < 0.05) (Fig. Adonitol b. b. Adequately recording the procedures and results of each test (25 pts): • I (or anyone else) ... Mycobacterium phlei Sporosarcina ureae Staphylococcus aureus Staphylococcus epidermidis Mycobacterium smegmatis doesn't need so many copies of the genes because it doesn't require the high production of proteins when it is growing slow, while Escherichia coli does. Immediately after the hydrogen peroxide solution to the presence of the pH indicator did not undergo a change in color and The test shows the bacterial decarboxylation and was not specific for the growth of Serratia Starch Hydrolysis ... -Media: skim milk agar-Enzyme: caseinase-Reagents: none-Results: clear areas next to growth indicate positive for casein. Positive. Gram stain of Serratia Positive for the fermentation of glucose to produce acid, but negative for The usually red colonies had a bluish tinge when seen against – Positive. to the surface of the tube. ALL GRAM NEGATIVE ORGANISMS. the bacterial decarboxylation of lysine which 롾 of ornithine, which results in the formation of an Oxidation Biochemical tests were performed and the results showed a bacterium belonging to the group Mycobacterium smegmatis. The organism is not capable of utilizing citrate in the form of its The colonies were pigmented only at room temperature or … The M. smegmatis ADHC was purified from M. smegmatis and the kinetic parameters of this enzyme showed that using NADPH as electron donor it … products. marcescens. a. Urease postive Organism: Klebsiella pneumoniae. Match. DTC agar was the enrichment media we chose to grow Serratia marcescens. Procedure /Method of Gelatin hydrolysis test. Does not ferment mannose and rhamnose. Test. ... Urease Test. The test can also be used to differentiate genera of gelatinase-producing bacteria such Serratia and Proteus from other members of the family Enterobacteriaceae. If you get these two pH indicators confused, you will have a difficult time interpreting test results. Biochemical Test of Mycobacterium tuberculosis. an intermediate in the production of butyl glycerol in the fermentation of Notes The quick growth rate of this microorganism is ideal for in-vitro testing, as other bacteria in this Genus may take several weeks to demonstrate growth. capable of producing pyruvic acid from the deamination of phenylalnine. Mycobacterium tuberculosis: Is the pathogen responsible for tuberculosis. served as a selective media. Abstract. shows a change in the pH from acidic to alkaline conditions. marcescens revealed that the organism was a gram negative bacteria, MacConkey's agar Biochemical tests were performed and the results showed a bacterium belonging to the group Mycobacterium smegmatis. jill_uebele. �b��Ң���E c�w٧�ˋj[?Bw^?����o�U+r)}vY��R7OϭpNg?՝�L��jQ=m�6��z�^\���;�� �PRJrpO֫j�,��p�,뭸�����j�#ٮ�e������o�d�i7u��]�7�jAM_�`��٧�Z4竧E-dv���?D!��o/5u�`7�K��d��)>~�1e�2�ݟW��f��}iV�m߯�Ͷ�|�6�{Z����*�P�f7_�j1���ךb������y{�}!�OQ�1���,���B)�my��P��L�O�wc��R��b`�*^)�Y��t���:�Q(����"�(�Ǣ�v����ul��!�ו����sNc�ɛcL��:�40� This indicted that the organism did not utilize (Ask instructor for results of this test if media is not available) Organism: Mycobacterium smegmatis II. The usually red colonies had a bluish tinge when seen against the background of the blue DTC agar. Pathogenic mycobacteria express a functional urease, but its role during infection has yet to be characterized. The organism is not The organism is not capable of fermenting dulcitol which results in the formation of acidic end DTC agar was the enrichment media we chose to grow Serratia marcescens. At room temperature, Serratia marcescens grew as bright, The motility stab showed that the organism was motile since it moved upward (Ask instructor for results of this test if media is not available.) Mycobacterium smegmatis: Found on skin and mucous membranes, non-pathogenic : Acid-fast, non-sporing, Gram-positive rod. Media: KH 2 PO 4 (0.5 g), MgSO> 4 *7H 2 0 (0.5 g), purified agar (20 g), distilled water (1000 ml). Glucose – Organism: Mycobacterium smegmatis . ... Mycobacterium smegmatis test results Mycobacterium smegmatis oxidase test results Download Here Free HealthCareMagic App to Ask a Doctor. Lactose – Klebsiella pneumoniae The colonies were pigmented only at room The organism was able to grow in both aerobic and Antimicrobial susceptibility … Since the gram stain of Serratia had been added to a sample of the organism, it produced effervescence. It was first reported in November 1884 by Lustgarten, who found a bacillus with the staining appearance of tubercle … After plating mycobacterium smegmatis on LB agar for 3 three days, I picked some clones and cultured bacterium in test tubes with LB medium with 0.05% tween 80 and 30ug/ml kanamycin. H2S Enterotube – The tests and their results Negative. Urease is an enzyme possessed by many Mycobacterium spp. Created by. The test detects the presence of aceylmethylcarbinol, Urease represents a critical virulence factor for some bacterial species through its alkalizing effect, which helps neutralize the acidic microenvironment of the pathogen. In an effort to improve diagnostic accuracy, whole-blood interferon release assays (IGRAs) have been commercially developed. 653 0 obj <>stream Does not ferment mannose and rhamnose. Sorbitol In microbiology, the phenotypic testing of mycobacteria uses a number of methods. Ornithine ALL GRAM NEGATIVE ORGANISMS Mycobacterium smegmatis urease test Mycobacterium smegmatis test that determines it Diagnosis of mycobacterium smegmatis ... Mycobacterium smegmatis oxidase test results Download Here Free HealthCareMagic App to Ask a Doctor. 630 0 obj <> endobj The ammonia released results in the alkalinisation and an increase in the pH of the medium turning the indicator pink. The organism does not ferment adonitol The change in the color of the indicator from pale yellow to purple Two classes of catalase, thermolabile and thermostable, appear in mycobacteria. The reaction results in the release of water and free oxygen (Palomino et al 2007). following Mycobacterium species gave positive results at 7 days: M. tuberculosis, M.kansasii, ... Asatisfactory urease test is needed to aid in ... semiquantitative catalase test results andposi-tive urease tests, so that the 93% of the M. Phenol red turns yellow below a pH of 6.8. Most of the possible unknown organisms will be mesophilic and will grow well at either temperature. Abstract. The presence was indicated by the development of a red color several M. smegmatis shares a number of morphological traits with M. tuberculosis including the distinctive waxy cell wall that provides a robust resistance to chemical disinfectants and sanitizers. In addition, urease serves as a nitrogen source provider for bacterial growth. Citrate – %PDF-1.5 %���� Lysine – the production of gas. Adequately recording the procedures and results of each test (25 pts): • I (or anyone else) ... Mycobacterium phlei Sporosarcina ureae Staphylococcus aureus Staphylococcus epidermidis Motility stab. Organism: Mycobacterium smegmatis . ALL GRAM NEGATIVE ORGANISMS. glossy red colonies on the TSA plate. Biochemical Test and Identification of Serratia marcescens. The agar was a general enrichment agar metabolize mannitol to produce acid, but gas was not produced. Sometimes the results of the tuberculin skin test are equivocal, particularly in persons who have been vaccinated with BCG or who live in areas where NTM are highly prevalent in the environment. TEST RESULTS. The organism is not capable of fermenting arabinose which results in the formation of acidic end production – Negative. Voges- Proskauer The organism does not have the enzyme urease Negative. temperature or about 25 degrees Celsius. Mycobacterium smegmatis urease test . The colonies were pigmented only at room temperature or about 25 degrees Celsius. Fermentation tests. h�Ԙ�k�6��=���>m8���m(��+��v}���wf��ʎ���p-y}�vV�!�3V�%�B��hPJ� anaerobic tubes. culture revealed that it was a gram negative rod. Organism: Mycobacterium phlei b. Ferments mannose and rhamnose Organism: Mycobacterium phlei. that is capable of hydrolyzing urea to ammonia. Negative. The most-commonly used phenotypic tests to identify and distinguish Mycobacterium strains and species from each other are described below.. Tests Acetamide as sole C and N sources. from pale yellow to purple. which results in the formation of acidic end products. to metabolize tryptophan. ted taxonomists to test directly the genetic relationships amongbacteria. change in color several minutes after the reagent had been added. Cell structure and metabolism. The lactose tubes that were originally red due uxZ��06�)����q� ��f�#(#�͙P��3 DC�K�qiqJm�)�U��ޒ%x���� ��B�6 �ƾ���������0-v'&Y��`��-�h�p�,S�i�ݴ�и4#��k��n�I1��Ѹ'E�*w,om��ʝ�۳���q�~��v��k�^���OC������@�H8��'�7��r'�^j�9�РC:�"S���Ô��DR�)|m���l@�7���m_]��U��V�Q����oT�/�4�o5�o��_��v�f�ߵ�ï5��_��6Y����fJ$�8hd�c��E��?�S����)?�߰S�������7Wґ�7�C��>�=sX��y�(N�q�ni��Kh̅��B�Oڰ(Na̐Na.|��0�+����x��}UNSAWR����)��t�8��e(t� The gene encoding of an alcohol dehydrogenase C (ADHC) from Mycobacterium smegmatis was cloned and sequenced. TEST RESULTS. The reaction results in the release of water and free oxygen (Palomino et al 2007). b. �in|и=-rG|�e���`��DP~ w��;�h�����D��"7���%4��i���C��ra��=��3�s���Qb������������_ �L � NOTE: Methyl red differs from Phenol red (which is used in the fermentation test and the MSA plates) in that it is yellow at pH 6.2 and above and red at pH 4.4 and below. The gene encoding of an alcohol dehydrogenase C (ADHC) from Mycobacterium smegmatis was cloned and sequenced. – Negative. 5 % NaCl Tolerance = Negative (-ve), 68°C Catalase Test = Negative (-ve), Acid Fast Stain = Positive (-ve), Acid Phosphatase = Negative (-ve), Amidase Test = Positive (-ve), Arylsulphatase Test = Negative (-ve). products. This indicates that the organism is not Positive. Principle: Many Mycobacterium species possess urease enzyme that hydrolyzes urea to form carbon dioxide and ammonia. If an organism which is red at 25 o C but only slightly pink at 37 o C, it is a mistake to presume the organism … 643 0 obj <>/Filter/FlateDecode/ID[<2E3301542F7F9B44820E42E454E190F4>]/Index[630 24]/Info 629 0 R/Length 72/Prev 462517/Root 631 0 R/Size 654/Type/XRef/W[1 2 1]>>stream Premium Questions. Mycobacterium smegmatis. which results in the formation of acidic end products. Serratia marcescens was able to that can hydrolyse urea to form ammonia and carbon dioxide. Terms in this set (23) ... urease positive, grows on MSA, does not ferment mannitol. �. marcescens. Mycobacterium tuberculosis: Is the pathogen responsible for tuberculosis. Mycobacterium smegmatis-Acid fast - Saprophyte (feeds on decaying matter)-gram positive rod. The sample on the cotton swab did not undergo a Klebsiella pneumoniae there was no gas production. Optimal Growth Temperature: Determine the optimum growth temperature by growing the unknown at 25 o C and 37 o C and note where the organism grows best. The genome was sequenced in November 29, 2006 by the J. Craig Venter Institute (9). indicates that the organism does not have the enzyme tryptophanase For strain 1, a slight decrease in turbidity is observed in the tube containing the bile salts (2nd from left), but the contents are almost as turbid as the control tube (far left); therefore, strain 1 is not S. pneumoniae. The rapid urease test confirmed a significantly lower rate of H. pylori infection in the recombinant Mycobacterium group (50%) than in the normal control (100%) and M. smegmatis (90%) groups (P < … h�bbd``b`�$ڀ�>�`}$X�@� �DH�!���~��iL�L� �!����` $� Acid was produced causing a change in the color of the pH Guinea pig, died 22 days after inoculation with butter sample No 15 GP 60 ATCC 19420; GP 60 Originally Deposited as Mycobacterium butyricum Isolated by F Griffith, Ministry of Health, 1920.Redeposited by ATCC 1932 PRE:FR All isolates used in this investiga-tion gave standard urease test results which agreedwiththeliterature (3, 5). Ofthe 16 mycobacterial species in-cludedinthis study, 12(98isolates) arereported to beurease positive, and4 are urease negative (42 isolates). %%EOF 2.2.3 Catalase test This is an antioxidan t enzyme responsible for eliminatin g molecules of hydrogen peroxide from the cells that are produced during respiration. ... Urease Test. Rapid Urease Test (RUT) The rapid urease test (RUT) is a popular diagnostic test for diagnosis of Helicobacter pylori. It is also known as the CLO test (Campylobacter-like organism test). DTC agar was the enrichment Growth on Macconkey's agar. Urea – Mycobacterium smegmatis is an acid-fast bacterial species in the phylum Actinobacteria and the genus Mycobacterium.It is 3.0 to 5.0 µm long with a bacillus shape and can be stained by Ziehl-Neelsen method and the auramine-rhodamine fluorescent method. endstream endobj 631 0 obj <>/Metadata 93 0 R/PageLayout/OneColumn/Pages 626 0 R/StructTreeRoot 119 0 R/Type/Catalog>> endobj 632 0 obj <>/Font<>>>/Rotate 0/StructParents 0/Type/Page>> endobj 633 0 obj <>stream Arabinose II. Indol formation – Negative. endstream endobj 635 0 obj <>stream PLAY. h�b```�t6�(Ad`f`B��f�%+.��q��3o���%;�X~����?��A~���s`����5m�����8:":,:8::8�A�� ������ b`{����X ,����S���~`,�N|�Y�U�-��������"���9H=*���xv��@��� J� capable to reducing sulfur containing products. Since all of the test strains showed Gram-positiveness (character 1), positive catalase (character 9), positive growth on 62.5 ,g/ml NHOOH medium (character 16), no acid production from raffinose (character Growth On T- Soy agar. The protein encoded by this gene has 78% identity with Mycobacterium tuberculosis and Mycobacterium bovis BCG ADHC. Gram-positive Rod Nitrate - Catalase + Bacillus pumilis otilit + Catalase - Lactobacillus bulgaricus Nitrate + Catalase + Amylase - Amylase + Bacillus subtilis Mycobacterium smegmatis If visible, definitive feature is acid-fast cell wall Colonies tend to be white or cream in color, very dry If visible, definitive feature is endospore Also positive for fat hydrolysis Colonies tend to be … Dulcitol Gravity. Results of the bile solubility test are shown for two different strains of bacteria. a. Urease … Mycobacterium a. Ferments mannose and rhamnose.